Title: DNA fluorocode: A single molecule, optical map of DNA with nanometre resolution
Authors: Neely, Robert K ×
Dedecker, Peter
Hotta, Jun-ichi
Urbanaviciute, Giedre
Klimasauskas, Saulius
Hofkens, Johan #
Issue Date: Oct-2010
Publisher: Royal soc chemistry
Series Title: Chemical science vol:1 issue:4 pages:453-460
Abstract: We present a new method for single-molecule optical DNA mapping using an exceptionally dense, yet sequence-specific coverage of DNA with a fluorescent probe. The method employs a DNA methyltransferase enzyme to direct the DNA labelling, followed by molecular combing of the DNA onto a polymer-coated surface and subsequent sub-diffraction limit localization of the fluorophores. The result is a 'DNA fluorocode'; a simple description of the DNA sequence, with a maximum achievable resolution of less than 20 bases, which can be read and analyzed like a barcode. We demonstrate the generation of a fluorocode for genomic DNA from the lambda bacteriophage using a DNA methyltransferase, M.Hhal, to direct fluorescent labels to four-base sequences reading 5'-GCGC-3'. A consensus fluorocode that allows the study of the DNA sequence at the level of an individual labelling site can be generated from a handful of molecules.
ISSN: 2041-6520
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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