Title: Molecular cloning of murine interferon gamma (MuIFN-gamma) cDNA and its expression in heterologous mammalian cells
Authors: Dijkmans, R ×
Volckaert, Guido
Van Damme, Jozef
De Ley, Marc
Billiau, Alfons
De Somer, P #
Issue Date: Jan-1985
Series Title: Journal of interferon research vol:5 issue:3 pages:511-20
Abstract: A complete cDNA clone of murine interferon-gamma (MuIFN-gamma) was obtained by recombining two appropriate segments from partial cDNA clones originally identified by colony hybridization with rat IFN-gamma chromosomal gene fragments as probes. An expression vector was constructed in which the cDNA was placed under control of the SV40 early promoter. Transient expression of MuIFN-gamma was obtained by transformation of COS-1 cells. Subsequently, this interferon expression unit was linked to a vector containing a dihydrofolate reductase (DHFR) modular gene and used to transform DHFR(-)-CHO cells. Cell clones were selected that constitutively produce an interferon activity which by several criteria was found to be indistinguishable from natural, splenocyte-derived MuIFN-gamma.
ISSN: 0197-8357
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Academic Center for General Practice
Laboratory of Molecular Immunology (Rega Institute)
Biochemistry, Molecular and Structural Biology Section
Laboratory of Immunobiology (Rega Institute)
× corresponding author
# (joint) last author

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