Title: Analysis of murine C-type virus structural proteins by rocket and crossed immunoelectrophoresis
Authors: Georgiades, J A ×
Billiau, Alfons
Verbruggen, R
de Somer, P #
Issue Date: Jan-1977
Series Title: Journal of Immunological Methods vol:17 issue:1-2 pages:177-87
Abstract: We have examined the structural proteins of Rauscher murine leukemia virus (R-MuLV) by means of rocket immunoelectrophoresis and crossed immunoelectrophoresis, using polyspecific antisera to Tween/ether-disrupted purified R-MuLV. Fifteen different precipitation lines were recognized in virus lysates. Using five reference antisera prepared to purified R-MuLV-structural proteins, the precipitation lines of p 10, p 15, p 30 and gp 69/70 were identified. These techniques, although less sensitive than radioimmunoassay, have several advantages, such as simplicity, direct control of precipitation reactions and possibility of using crude antigen preparations.
ISSN: 0022-1759
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Immunobiology (Rega Institute)
× corresponding author
# (joint) last author

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