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Title: Cloning of cDNA coding for human tissue-type plasminogen activator and its expression in Escherichia coli
Authors: Harris, T J ×
Patel, T
Marston, F A
Little, S
Emtage, J S
Opdenakker, Ghislain
Volckaert, Guido
Rombauts, Wilfried
Billiau, Alfons
De Somer, P #
Issue Date: Jun-1986
Series Title: Molecular biology & medicine vol:3 issue:3 pages:279-92
Abstract: cDNA clones of the mRNA coding for tissue-type plasminogen activator (t-PA) have been obtained and their nucleotide sequences compared to those reported previously. A gene coding for t-PA has been reconstructed and inserted into vectors for expression in prokaryotic cells. Relatively high levels of t-PA accumulated in inclusion bodies in Escherichia coli containing an optimized expression plasmid, but only a small proportion of the insoluble protein was recovered as active enzyme using a variety of solubilization procedures.
ISSN: 0735-1313
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Biochemistry Section (Medicine) (-)
Laboratory of Immunobiology (Rega Institute)
× corresponding author
# (joint) last author

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