Title: A molecular toolbox to estimate the number and diversity of Variovorax in the environment: application in soils treated with the phenylurea herbicide linuron
Authors: Bers, Karolien ×
Sniegowski, Kristel
Albers, Pieter
Breugelmans, Philip
Hendrickx, Larissa
De Mot, René
Springael, Dirk #
Issue Date: Apr-2011
Publisher: Published by Elsevier Science Publishers on behalf of the Federation of European Microbiological Societies
Series Title: FEMS Microbiology Ecology vol:76 issue:1 pages:14-25
Abstract: Real-time PCR and PCR-denaturing gradient gel electrophoresis (DGGE) approaches that specifically target the Variovorax 16S rRNA gene were developed to estimate the number and diversity of Variovorax in environmental ecosystems. PCR primers suitable for both methods were selected as such that the enclosed sequence showed maximum polymorphism. PCR specificity was maximized by combining PCR with a targeted endonuclease treatment of template DNA to eliminate 16S rRNA genes of the closely related Acidovorax. DGGE allowed the grouping of PCR amplicons according to the phylogenetic grouping within the genus Variovorax. The toolbox was used to assess the Variovorax community dynamics in agricultural soil microcosms (SMs) exposed to the phenylurea herbicide linuron. Exposure to linuron resulted in an increased abundance within the Variovorax community of a subgroup previously linked to linuron degradation through cultivation-dependent isolation. SMs that were treated only once with linuron reverted to the initial community composition 70 days after linuron exposure. In contrast, SMs irrigated with linuron on a long-term base showed a significant increase in Variovorax number after 70 days. Our data support the hypothesis that the genus Variovorax is involved in linuron degradation in linuron-treated agricultural soils.
ISSN: 0168-6496
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division Soil and Water Management
Department of Materials Engineering - miscellaneous
Centre of Microbial and Plant Genetics
× corresponding author
# (joint) last author

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