Title: SIFamide is a highly conserved neuropeptide: a comparative study in different insect species
Authors: Verleyen, Peter
Huybrechts, Jurgen
Baggerman, Geert
Van Lommel, Alfons
De Loof, Arnold
Schoofs, Liliane # ×
Issue Date: Jul-2004
Publisher: Academic Press
Series Title: Biochemical and Biophysical Research Communications vol:320 issue:2 pages:334-341
Abstract: Neb-LFamide or AYRKPPFNGSLFamide was originally purified from the grey flesh fly Neobellieria bullata as a myotropic neuropeptide. We studied the occurrence of this peptide and its isoforms in the central nervous system of different insect species by means of whole mount fluorescence immunohistochemistry, mass spectrometry, and data mining. We found that both sequence and immunoreactive distribution pattern are very conserved in the studied insects. In all species and stages we counted two pairs of immunoreactive cells in the pars intercerebralis. These cells projected axons throughout the ventral nerve cord. In the adult CNSs they formed a large number of immunoreactive varicosities as well. Mass spectrometry and data mining revealed that SIFamide exists in two isoforms: [G1]-SIFamide and [A1]-SIFamide. In addition, the SIFamide joining peptide is relatively well conserved throughout arthropod species. The conserved presence of two cysteine residues, separated by six amino acid residues, allows the formation of disulphide bridges.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Translational Cell & Tissue Research
Department of Biology - miscellaneous
Microbial and Molecular Systems - miscellaneous
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Verleyen et al._2004_Biochem Biophys Res Comm_vol320_p334-341.pdf Published 378KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science