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Title: Synthesis and spectroscopic characterisation of BODIPY based fluorescent off-on indicators with low affinity for calcium
Authors: Basarić, Nikola ×
Baruah, Mukulesh
Qin, Wenwu
Metten, Bert
Smet, Mario
Dehaen, Wim
Boens, Noël #
Issue Date: Aug-2005
Publisher: Royal Society of Chemistry
Series Title: Organic & Biomolecular Chemistry vol:3 issue:15 pages:2755-2761
Abstract: Two fluorescent off-on Ca2+ indicators based on APTRA (o-aminophenol-N,N,O-triacetic acid) as low-affinity ligand for Ca2+ and BODIPY(4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) as a fluorophore were synthesized. The new BODIPY-APTRA compounds absorb in the visible spectrum, with absorption maxima from 505 nm to 570 nm, and have fluorescence spectra that span the visible spectrum, with emission maxima ranging from 525 nm to 625 nm dependent on the substituents at the alpha-positions to the nitrogen atoms. The indicators show a large increase of the fluorescence quantum yield upon increasing Ca2+ concentration. The ground-state dissociation constants Kd estimated at 20 degrees C in 100 mM KCl aqueous buffered solution, pH 7.20, for the two complexes with Ca2+ were found to be around 100 microM.
ISSN: 1477-0520
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
Molecular Design and Synthesis
Polymer Chemistry and Materials
× corresponding author
# (joint) last author

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