Author:

Keywords:

Science & Technology, Life Sciences & Biomedicine, Hematology, cell line, leukemia, thymocyte, interleukin, mouse, ACUTE LYMPHOBLASTIC-LEUKEMIA, CHRONIC MYELOID-LEUKEMIA, TYROSINE KINASES, MUTATION, PROTEIN, NOTCH1, INHIBITION, ACTIVATION, RESISTANCE, THERAPY, Animals, Apoptosis, Blotting, Western, CD4 Antigens, CD8 Antigens, Carcinogens, Cell Line, Cell Line, Tumor, Cell Proliferation, Female, Fusion Proteins, bcr-abl, HEK293 Cells, Humans, In Situ Hybridization, Fluorescence, Interleukin-7, Janus Kinase 1, Karyotyping, Mice, Mice, Inbred BALB C, Mutation, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma, Receptor, Notch1, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, T-Lymphocytes, 1102 Cardiorespiratory Medicine and Haematology, Immunology, 3201 Cardiovascular medicine and haematology

Abstract:

The mouse pro-B cell line Ba/F3 has gained major interest as a model system to investigate oncogenic tyrosine kinases and to determine the efficacy of kinase inhibitors. While Ba/F3 cells are suitable to study oncogenic kinases derived from various cell types, the signaling networks in Ba/F3 cells are B-cell specific. We have established a mouse CD4+CD8+ double positive T-cell line (named MOHITO, for MOuse Hematopoietic Interleukin-dependent cell line of T-cell Origin) that has many features of human T-cell acute lymphoblastic leukemia (Notch1 and Jak1 mutation, TCR rearrangement) and is dependent on interleukin-7. The MOHITO cell line can be transformed to cytokine independent proliferation by BCR-ABL1 or mutant JAK1. This mouse T-cell line is a novel model system to investigate protein signaling and inhibition in a T-cell specific context and is a valuable tool to study and verify oncogenic capacity of mutations in the kinome and phosphatome in T-cell malignancies.