Title: Biodistribution and photodynamic effects of PVP-hypericin using multi-cellular spheroids composed of normal human urothelial and T24 transitional cell carcinoma cells
Authors: Vandepitte, Joachim
Van Cleynenbreugel, Ben
Lerut, Evelyne
Van Poppel, Hendrik
de Witte, Peter # ×
Issue Date: Jan-2011
Publisher: Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society
Series Title: Journal of Biomedical Optics vol:16 issue:1
Article number: 018001
Abstract: Polyvinylpyrrolidone (PVP)-hypericin is a potent photosensitizer that is used in the urological clinic to photodiagnose with high-sensitivity nonmuscle invasive bladder cancer (NMIBC). We examined the differential accumulation and therapeutic effects of PVP-hypericin using spheroids composed of a human urothelial cell carcinoma cell line (T24) and normal human urothelial (NHU) cells. The in vitro biodistribution was assessed using fluorescence image analysis of 5-μm cryostat sections of spheroids that were incubated with PVP-hypericin. The results show that PVP-hypericin accumulated to a much higher extent in T24 spheroids as compared to NHU spheroids, thereby reproducing the clinical situation. Subsequently, spheroids were exposed to different PDT regimes with a light dose ranging from 0.3 to 18J∕cm(2). When using low fluence rates, only minor differences in cell survival were seen between normal and malignant spheroids. High light fluence rates induced a substantial difference in cell survival between the two spheroid types, killing ∼80% of the cells present in the T24 spheroids. It was concluded that further in vivo experiments are required to fully evaluate the potential of PVP-hypericin as a phototherapeutic for NMIBC, focusing on the combination of the compound with methods that enhance the oxygenation of the urothelium.
ISSN: 1083-3668
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory for Pharmaceutical Biology (-)
Urology Section (-)
Translational Cell & Tissue Research
× corresponding author
# (joint) last author

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