The attitude of patients with reproductive disorders regarding the use of Non-Human Primates (NHPs) in preclinical reproductive research and its determinants were examined. A survey was conducted with 299 patients with fertility problems and/or endometriosis in a European Fertility centre (RR= 80%). The main outcome measure was the attitude towards reproductive research on NHPs. In total, 70.6% accepts and 29.4% rejects NHP research. Factors significantly positively related to acceptance: confidence in researchers and previous pregnancy. Factors significantly negatively related to acceptance: having a pet, membership of a nature organization, vegetarian and having lived abroad. To the best of our knowledge this study is the first on the patients’ perspective on NHP research. The majority of the patients accept reproductive research on NHPs. Trust in researchers was the most important positively related factor, therefore, researchers are advised to actively try to gain the trust of patients and the public.