Title: Novel nephelometric assay for measurement of complement 3d
Authors: Bossuyt, Xavier ×
Sneyers, Lutgarde
Mariën, Godelieve
Vranken, Guido #
Issue Date: Jan-2002
Series Title: Annals of clinical biochemistry vol:39 issue:Pt 1 pages:34-8
Abstract: BACKGROUND: This report describes a nephelometric assay for measuring complement 3d (C3d). METHODS: C3d was separated from C3 and the other C3 split products by polyethylene glycol precipitation. The supernatant containing C3d was then measured by rate nephelometry in a Beckman Immage rate nephelometer using a specific antibody. Calibration was performed with dilutions of a standard, which was obtained by incubating a serum pool at 37 degrees C for 7 days. This incubation step allowed the activation and breakdown of C3 into C3d. RESULTS: The assay was linear across the normal and pathological range. Precision studies showed within-run and between-run coefficients of variation of < 5% and < 6%, respectively. The nephelometric results are comparable with those obtained by radial immunodiffusion. Neither haemoglobin nor lipids interfere with the assay.
ISSN: 0004-5632
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical Bacteriology and Mycology
Experimental Laboratory Immunology
× corresponding author
# (joint) last author

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