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Title: Exploring the aneugenic and clastogenic potential in the nanosize range: A549 human lung carcinoma cells and amorphous monodisperse silica nanoparticles as models
Authors: Gonzalez, Laetitia ×
Thomassen, Leen
Plas, Gina
Rabolli, Virginie
Napierska, Dorota
Decordier, Ilse
Roelants, Mathieu
Hoet, Peter
Kirschhock, Christine
Martens, Johan
Lison, Dominique
Kirsch-Volders, Micheline #
Issue Date: Dec-2010
Publisher: Informa Healthcare
Series Title: Nanotoxicology vol:4 issue:4 pages:382-395
Abstract: We explored how to assess the genotoxic potential of nanosize particles with a well validated assay, the in vitro cytochalasin-B micronucleus assay, detecting both clastogens and aneugens. Monodisperse Stober amorphous silica nanoparticles (SNPs) of three different sizes (16, 60 and 104 nm) and A549 lung carcinoma cells were selected as models. Cellular uptake of silica was monitored by ICP-MS. At non-cytotoxic doses the smallest particles showed a slightly higher fold induction of micronuclei (MNBN). When considering the three SNPs together, particle number and total surface area appeared to account for MNBN induction as they both correlated significantly with the amplitude of the effect. Using nominal or cellular dose did not show statistically significant differences. Likewise, alkaline comet assay and FISH-centromeric probing of MNBN indicated a weak and not statistically significant induction of oxidative DNA damage, chromosome breakage and chromosome loss. This line of investigation will contribute to adequately design and interpret nanogenotoxicity assays.
ISSN: 1743-5390
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Surface Chemistry and Catalysis
Occupational, Environmental and Insurance Medicine (-)
Youth Health (-)
Environment and Health - miscellaneous
Formerly "Departement Industriƫle Wetenschappen - TM M"
Microbial and Molecular Systems - miscellaneous
× corresponding author
# (joint) last author

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