Title: Detection of single nucleotide polymorphisms in the mannose-binding lectin gene using minor groove binder-DNA probes
Authors: Van Hoeyveld, Ernestina ×
Houtmeyers, Frans
Massonet, Caroline
Moens, Leen
Van Ranst, Marc
Blanckaert, Norbert
Bossuyt, Xavier #
Issue Date: Apr-2004
Series Title: Journal of Immunological Methods vol:287 issue:1-2 pages:227-230
Abstract: Structural point mutations in exon 1 at codons 52, 54 and 57 and a promotor polymorphism at -221 bp of the mannose-binding lectin (MBL) gene are associated with increased susceptibility to various infectious diseases. We developed a genotyping method based on the 5' nuclease (TaqMan) assay in combination with the use of minor-groove-binder (MGB) probes in screening for these mutations/polymorphisms. In contrast to conventional probes, MGB probes have a short length and can be used for detection of mutations that are in close proximity to each other, as is the case for the structural mutations in exon 1 of the MBL gene. Results obtained with the 5' nuclease assay using MGB probes were identical with results obtained with classical techniques such as restriction fragment length polymorphism, allele-specific PCR, and sequencing. In conclusion, the 5' nuclease assay using MGB probes is useful for large-scale screening of point mutations/polymorphisms, even when they are in close proximity.
ISSN: 0022-1759
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical Bacteriology and Mycology
Laboratory of Clinical and Epidemiological Virology (Rega Institute)
Experimental Laboratory Immunology
Department of Imaging & Pathology - miscellaneous
Laboratory of Clinical Immunology
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science