Title: The low molecular weight cyclin E isoforms augment angiogenesis and metastasis of human melanoma cells in vivo
Authors: Bales, Elise ×
Mills, Lisa
Milam, Nancy
McGahren-Murray, Mollianne
Bandyopadhyay, Debdutta
Chen, Dahu
Reed, Jon A
Timchenko, Nikolai
van den Oord, Joost
Bar-Eli, Menashe
Keyomarsi, Khandan
Medrano, Estela E #
Issue Date: Feb-2005
Series Title: Cancer Research vol:65 issue:3 pages:692-7
Abstract: Immunohistochemical analysis has consistently shown that cyclin E is up-regulated in human malignant melanomas in vivo. Here we analyzed such expression in more detail and show that cyclin E is overexpressed and present in low molecular weight (LMW) isoforms in metastatic melanoma and in a subset of primary invasive melanoma tumor tissues, but not in benign nevi. Human metastatic melanoma cell lines, but not normal melanocytes, also expressed the LMW cyclin E forms. The biological significance of these findings was established by showing that overexpression of two LMW cyclin E forms named cyclin E truncated 1 [cyclinE(T1)] and cyclin E truncated 2 [cyclinE(T2)] in a low tumorigenic and non-metastatic primary cutaneous melanoma cell line generated angiogenic tumors with prominent perineural invasion compared with full-length cyclin E. In addition, cyclin E(T1)- and cyclin E(T2)-expressing melanoma cells displayed a dramatic increase in the incidence and number of metastases in an experimental lung metastasis assay. Together, these results indicate that the LMW cyclin E forms are functional and likely act as regulators of human melanoma tumor progression and invasion.
ISSN: 0008-5472
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Translational Cell & Tissue Research
× corresponding author
# (joint) last author

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