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Title: Atomic structure of vimentin coil 2
Authors: Nicolet, Stefan
Herrmann, Harald
Aebi, Ueli
Strelkov, Sergei # ×
Issue Date: May-2010
Publisher: Academic press inc elsevier science
Series Title: Journal of structural biology vol:170 issue:2 pages:369-376
Abstract: Intermediate filaments (IFS) are essential cytoskeletal components in metazoan cells. They assemble from elementary dimers that are built around the central alpha-helical coiled-coil rod domain representing the IF 'signature'. The rod consists of two similarly-sized parts, coil 1 and coil 2, connected by a non-alpha-helical linker L12. Coil 2 is absolutely conserved in length across all IF types and was initially predicted to consist of a short coiled-coil segment 2A based on a heptad pattern of hydrophobic residues, another linker L2 and a coiled-coil segment 2B. Here we present the crystal structure of human vimentin fragment including residues 261-335 i.e. approximately the first half of coil 2. The N-terminal part of this fragment reveals a parallel alpha-helical bundle characterized by 3.5 consecutive hendecad repeats. It is immediately followed by a regular left-handed coiled coil. The distinct non-helical linker L2 is therefore not observed. Together with the previously determined crystal structure of the major part of segment 2B (Strelkov et al., 2002), we can now build a complete atomic model of the 21 nm long vimentin coil 2 dimer being a relatively rigid rod. (C) 2010 Elsevier Inc. All rights reserved.
URI: 
ISSN: 1047-8477
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biocrystallography
× corresponding author
# (joint) last author

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