Title: Development and performance of a quantitative PCR for the enumeration of Bdellovibrionaceae
Authors: Van Essche, Mark
Sliepen, Isabelle
Loozen, Gitte
Van Eldere, Johan
Quirynen, Marc
Davidov, Y
Jurkevitch, E
Boon, N
Teughels, Wim # ×
Issue Date: Aug-2009
Publisher: Wiley-blackwell publishing, inc
Series Title: Environmental microbiology reports vol:1 issue:4 pages:228-233
Abstract: Quantification of Bdellovibrio-and-like organisms (BALOs) by microbial culturing has a number of substantial drawbacks. Therefore a quantitative PCR (qPCR) assay was designed for the culture-independent enumeration of the Bdellovibrionaceae. After optimization, the dynamic range of the qPCR assay was assessed, the specificity was evaluated and a comparison with quantitative microbial culturing was made. To evaluate the suitability of the qPCR assay for analysing environmental samples, fresh water samples were investigated by microbial culturing and by the qPCR assay. The results revealed a substantial difference between the two techniques and indicate that most Bdellovibrionaceae cells are left undetected in environmental samples when only current microbial culturing techniques are used. The application of this new technique is therefore likely to confirm the hitherto underestimated sizes and roles of predatory bacterial populations in nature.
ISSN: 1758-2229
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Periodontology
Laboratory for Experimental and Clinical Microbiology (-)
Laboratory of Clinical Bacteriology and Mycology
× corresponding author
# (joint) last author

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