Title: Second international collaborative study evaluating performance characteristics of methods measuring the von Willebrand factor cleaving protease (ADAMTS-13)
Authors: Tripodi, A ×
Peyvandi, F
Chantarangkul, V
Palla, R
Afrasiabi, A
Canciani, M. T
Chung, D. W
Ferrari, S
Fujimura, Y
Karimi, M
Kokame, K
Hovinga, J. A. Kremer
Laemmle, B
De Meyer, Simon
Plaimauer, B
Vanhoorelbeke, Karen
Varadi, K
Mannucci, P. M #
Issue Date: Sep-2008
Publisher: Blackwell publishing
Series Title: Journal of thrombosis and haemostasis vol:6 issue:9 pages:1534-1541
Abstract: Background: Over the last 4 years ADAMTS-13 measurement underwent dramatic progress with newer and simpler methods. Aims: Blind evaluation of newer methods for their performance characteristics. Design: The literature was searched for new methods and the authors invited to join the evaluation. Participants were provided with a set of 60 coded frozen plasmas that were prepared centrally by dilutions of one ADAMTS-13-deficient plasma (arbitrarily set at 0%) into one normal-pooled plasma (set at 100%). There were six different test plasmas ranging from 100% to 0%. Each plasma was tested 'blind' 10 times by each method and results expressed as percentage vs. the local and the common standard provided by the organizer. Results: There were eight functional and three antigen assays. Linearity of observed-vs.-expected ADAMTS-13 levels assessed as r(2) ranged from 0.931 to 0.998. Between-run reproducibility expressed as the (mean) CV for repeated measurements was below 10% for three methods, 10-15% for five methods and up to 20% for the remaining three. F-values (analysis of variance) calculated to assess the capacity to distinguish between ADAMTS-13 levels (the higher the F-value, the better the capacity) ranged from 3965 to 137. Between-method variability (CV) amounted to 24.8% when calculated vs. the local and to 20.5% when calculated vs. the common standard. Comparative analysis showed that functional assays employing modified von Willebrand factor peptides as substrate for ADAMTS-13 offer the best performance characteristics. Conclusions: New assays for ADAMTS-13 have the potential to make the investigation/management of patients with thrombotic microangiopathies much easier than in the past.
ISSN: 1538-7933
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Chemistry, Campus Kulak Kortrijk
Faculty of Science, Campus Kulak Kortrijk
× corresponding author
# (joint) last author

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