Title: Micellar electrokinetic chromatography method development for determination of impurities in Ritonavir
Authors: Carvalho, Alexandre Zatkovskis
El-Attug, Mohamed Nouri
Zayed, Sahar Ebrahem
Van Hove, Els
Van Duppen, Joost
Hoogmartens, Jos
Van Schepdael, Ann # ×
Issue Date: Dec-2010
Publisher: Pergamon Press
Series Title: Journal of Pharmaceutical and Biomedical Analysis vol:53 issue:5 pages:1210-1216
Abstract: Ritonavir is a synthetic peptidomimetic human immunodeficiency virus (HIV) protease inhibitor employed in the treatment of AIDS since 1996. Synthetic precursors are potential impurities in the final product. In the present work a micellar electrokinetic chromatography (MEKC) method for the separation of Ritonavir from three available synthetic precursors was developed. The optimized separation is performed in a background electrolyte composed of sodium tetraborate (pH 9.6: 15 mM) containing sodium dodecylsulfate (30 mM) and acetonitrile (18%, v/v) Mass spectrometry was used to confirm the identity of the tested substances. Good repeatability was observed for migration time (RSD about 0.4%) and peak area (RSD about 0 8%). The limits of detection (LOD) obtained allow the determination of two of the impurities at levels as low as 0.005% m/m, and one at a level of 0.3% m/m. (C) 2010 Elsevier B.V All rights reserved.
ISSN: 0731-7085
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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