Title: Differential Endothelial Transcriptomics Identifies Semaphorin 3G as a Vascular Class 3 Semaphorin
Authors: Kutschera, Simone
Weber, Holger
Weick, Anja
De Smet, Frederik
Genove, Guillem
Takemoto, Minoru
Prahst, Claudia
Riedel, Maria
Mikelis, Constantinos
Baulande, Sylvain
Champseix, Catherine
Kummerer, Petra
Conseiller, Emmanuel
Multon, Marie-Christine
Heroult, Melanie
Bicknell, Roy
Carmeliet, Peter
Betsholtz, Christer
Augustin, Hellmut G # ×
Issue Date: Jan-2011
Publisher: Lippincott, Williams & Wilkins
Series Title: Arteriosclerosis, Thrombosis and Vascular Biology vol:31 issue:1 pages:151
Abstract: OBJECTIVE: To characterize the role of a vascular-expressed class 3 semaphorin (semaphorin 3G [Sema3G]). METHODS AND RESULTS: Semaphorins have been identified as axon guidance molecules. Yet, they have more recently also been characterized as attractive and repulsive regulators of angiogenesis. Through a transcriptomic screen, we identified Sema3G as a molecule of angiogenic endothelial cells. Sema3G-deficient mice are viable and exhibit no overt vascular phenotype. Yet, LacZ expression in the Sema3G locus revealed intense arterial vascular staining in the angiogenic vasculature, starting at E9.5, which was detectable throughout adolescence and downregulated in adult vasculature. Sema3G is expressed as a full-length 100-kDa secreted molecule that is processed by furin proteases to yield 95- and a 65-kDa Sema domain-containing subunits. Full-length Sema3G binds to NP2, whereas processed Sema3G binds to NP1 and NP2. Expression profiling and cellular experiments identified autocrine effects of Sema3G on endothelial cells and paracrine effects on smooth muscle cells. CONCLUSIONS: Although the mouse knockout phenotype suggests compensatory mechanisms, the experiments identify Sema3G as a primarily endothelial cell-expressed class 3 semaphorin that controls endothelial and smooth muscle cell functions in autocrine and paracrine manners, respectively.
ISSN: 1079-5642
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Vesalius Research Centre (-)
Switch Laboratory
Laboratory of Angiogenesis and Vascular Metabolism (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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