Title: Laboratory Diagnosis of Specific Antibody Deficiency to Pneumococcal Capsular Polysaccharide Antigens
Authors: Jeurissen, Axel
Moens, Leen
Raes, Marc
Wuyts, Greet
Willebrords, Luc
Sauer, Kate
Proesmans, Marijke
Ceuppens, Jan
De Boeck, Kris
Bossuyt, Xavier # ×
Issue Date: Mar-2007
Publisher: P.B. Hoeber
Series Title: Clinical Chemistry vol:53 issue:3 pages:505-510
Abstract: BACKGROUND: Measurement of postimmunization antibody response to pneumococcal capsular polysaccharide (caps-PS) is the standard method to identify deficiency of antipolysaccharide antibody production. However, no standardized criteria have been defined for classification of patients into responders or nonresponders to caps-PS. METHODS: We vaccinated 37 healthy children and 39 healthy adults with Pneumovax((R)) and measured the anti-caps-PS antibody response to 5 serotypes. We also measured antipneumococcal antibody titers in 82 patients with increased susceptibility to airway infection. The assay was performed according to the 3rd-generation assay format. RESULTS: The lower 5th percentile (cutoff) concentrations for the postimmunization antibody titer in healthy individuals were 0.67 mg/L, 0.45 mg/L, 0.46 mg/L, 0.31 mg/L, and 1.04 mg/L for serotypes 3, 4, 9N, 18C, and 19F, respectively. In 96% of healthy individuals, antibody responses higher than the cutoff concentration were seen for at least 3 of the 5 serotypes. Nine of 82 patients (11%) failed to mount an adequate antibody response for at least 4 of the 5 serotypes tested, whereas only 1 control (1.3%) failed to do so. CONCLUSION: The cutoffs for antibody responses to caps-PS identified in this study appear useful for identifying individuals with an inadequate response to vaccine.
ISSN: 0009-9147
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical Bacteriology and Mycology
Pediatric Pulmonology Section (-)
Laboratory of Clinical Immunology
Experimental Laboratory Immunology
× corresponding author
# (joint) last author

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