Title: Specific magnetic isolation for direct detection of HPV16
Authors: Peeters, Sara ×
Stakenborg, Tim
Colle, Frederik
Chengxun, Liu
Lagae, Liesbet
Van Ranst, Marc #
Issue Date: Apr-2012
Publisher: Springer-Verlag
Series Title: European Journal of Clinical Microbiology & Infectious Diseases vol:31 issue:4 pages:539-546
Abstract: Finding a suitable DNA purification system is vital for the success of many PCR based diagnostic tests. This report demonstrates the value of magnetic beads in combination with real-time PCR for the sequence-specific isolation and detection of episomal HPV16 DNA. In order to maximize the isolation, two purification procedures were evaluated. Compared to the indirect method, in which the target was magnetically labeled after being hybridized to the capture probes, much higher efficiencies were obtained by directly capturing the target using DNA functionalized beads. These higher efficiencies were obtained by carefully tuning the capture probe density on the beads. When modifying the beads with dual-biotinylated capture probes or introducing beads modified with different capture probes, the amount of HPV16 isolated from spiked clinical swab samples even increased further. This not only resulted in the use of dual-biotinylated capture probes in higher purification efficiencies, but also the thermostability of the DNA-bead linkage was found to improve. In summary, this study shows that DNA functionalized magnetic beads are very promising diagnostic tools as they allow for a specific, simple, and fast isolation and concentration of minute quantities of DNA from complex clinical samples.
ISSN: 0934-9723
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical and Epidemiological Virology (Rega Institute)
Solid State Physics and Magnetism Section
Department of Health and Technology - UC Leuven
× corresponding author
# (joint) last author

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