Author:

Keywords:

human Wa-like strains, Bangladeshi G1P[8] rotavirus, Bangladesh, rotavirus, rotavirus G1P[8], Science & Technology, Life Sciences & Biomedicine, Infectious Diseases, Rotavirus, Complete genome, Sequence, G1P[8], Wa-like, Vaccine, CLASSIFICATION, VP7, REASSORTMENT, INFANTS, LIVE, DS-1-LIKE, SEQUENCE, BELONG, ORIGIN, GENES, Amino Acid Sequence, Antigenic Variation, Diarrhea, Evolution, Molecular, Genome, Viral, Humans, Infant, Molecular Sequence Data, Phylogeny, Rotavirus Infections, Sequence Analysis, DNA, Species Specificity, Time Factors, 0604 Genetics, Microbiology, 3102 Bioinformatics and computational biology, 3105 Genetics, 3107 Microbiology

Abstract:

More than 120 variants of rotavirus strains with different VP7 (G type) and VP4 (P type) combinations are reported thus far. Among them Wa-like G1P[8] rotaviruses are the most common human strains worldwide. However, characterization of their entire genome complement is limited to a few old prototype strains, and no complete genome data for any G1P[8] strain isolated in the last decade are available. Both the currently licensed rotavirus vaccines Rotarix and RotaTeq possess the G1 and P[8] specificities. Therefore, comprehensive genetic information of the currently circulating G1P[8] strain is important to assess the impact of rotavirus vaccines on the circulating rotavirus strains. Here we report the complete genome sequence of a G1P[8] rotavirus strain Dhaka16-03 isolated in 2003 from a Bangladeshi child hospitalized with severe diarrhea. Based on a full-genome classification system, Dhaka16-03 was shown to posses the typical Wa-like genotype constellation: G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-E1-H1. The strain was phylogenetically more closely related to contemporary human rotavirus strains (isolated in the 2000s) with a range of G and P-genotypes than to those of the prototype G1P[8] strains. Since the vaccine strains are developed based on strains isolated several decades ago, it is important to know how much the vaccine strains differ from the currently circulating G1P[8] and other Wa-like strains. Our complete genome characterization of a recent G1P[8] strain will be helpful to assess the ongoing rotavirus vaccine trials and their implementation programs in the forthcoming years.