Title: Proteome analysis of multiple compartments in a mouse model of chemical-induced asthma
Authors: Haenen, Steven * ×
Vanoirbeek, Jeroen *
De Vooght, Vanessa
Maes, Evelyne
Schoofs, Liliane
Nemery, Benoit
Hoet, Peter
Clynen, Elke #
Issue Date: Nov-2010
Publisher: American Chemical Society
Series Title: Journal of Proteome Research vol:9 issue:11 pages:5868-5876
Abstract: Occupational asthma is the principal cause of work-related respiratory disease in the industrial world. Toluene-2,4-diisocyanate (TDI) is one of the most common respiratory sensitizers leading to occupational asthma. Using a mouse model of chemical-induced asthma we explored proteome changes in multiple compartments of mice sensitized and challenged with TDI or acetone-olive oil (AOO; vehicle). Airway reactivity to methacholine and a bronchoalveolar lavage (BAL) cell count was assessed in treated and control mice, 1 day after challenge. Subsequently, two-dimensional differential gel electrophoresis (2D-DIGE) was performed on auricular lymph nodes, bronchoalveolar lavage (BAL) and serum comparing TDI-treated and control mice. The differentially expressed proteins were identified by mass spectrometry and pathway analysis was performed. TDI-treated mice exhibit increased airway reactivity (2.6 fold increase) and a neutrophilic inflammation in the BAL fluid, compared to control mice. 2D-DIGE showed 53, 210 and 40 differentially expressed proteins in the auricular lymph nodes, BAL and serum of TDI-treated versus vehicle-treated mice, respectively. Several of the identified proteins could be linked with inflammation, neutrophil chemotaxis and/or oxidative stress. Physiologic and immunologic readouts of the asthmatic phenotype, such as inflammation, were confirmed in three compartments by several of the differentially expressed proteins via 2D-DIGE and computerized pathway analysis.
ISSN: 1535-3893
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Occupational, Environmental and Insurance Medicine (-)
Animal Physiology and Neurobiology Section - miscellaneous
Environment and Health - miscellaneous
* (joint) first author
× corresponding author
# (joint) last author

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