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Title: MccE Provides Resistance to Protein Synthesis Inhibitor Microcin C by Acetylating the Processed Form of the Antibiotic
Authors: Novikova, Maria
Kazakov, Teymur
Vondenhoff, Gaston
Semenova, Ekaterina
Rozenski, Jef
Metlytskaya, Anastasija
Zukher, Inna
Tikhonov, Anton
Van Aerschot, Arthur
Severinov, Konstantin # ×
Issue Date: Apr-2010
Publisher: Amer soc biochemistry molecular biology inc
Series Title: Journal of biological chemistry vol:285 issue:17 pages:12662-12669
Abstract: The heptapeptide-nucleotide microcin C (McC) is a potent inhibitor of enteric bacteria growth. McC is excreted from producing cells by the MccC transporter. The residual McC that remains in the producing cell can be processed by cellular amino-peptidases with the release of a non-hydrolyzable aspartyl-adenylate, a strong inhibitor of aspartyl-tRNA synthetase. Accumulation of processed McC inside producing cells should therefore lead to translation inhibition and cessation of growth. Here, we show that a product of another gene of the McC biosynthetic cluster, mccE, acetylates processed McC and converts it into a non-toxic compound. MccE also makes Escherichia coli resistant to albomycin, a Trojan horse inhibitor unrelated to McC that, upon processing, gives rise to a serine coupled to a thioxylofuranosyl pyrimidine, an inhibitor of seryl-tRNA synthetase. We speculate that MccE and related cellular acetyltransferases of the Rim family may detoxify various aminoacyl-nucleotides, either exogenous or those generated inside the cell.
URI: 
ISSN: 0021-9258
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

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