Title: Mechanism of Na+ binding to thrombin resolved by ultra-rapid kinetics
Authors: Gianni, Stefano
Ivarsson, Ylva
Bah, Alaji
Bush-Pelc, Leslie A
Di Cera, Enrico # ×
Issue Date: Dec-2007
Publisher: North-Holland Pub. Co.
Series Title: Biophysical Chemistry vol:131 issue:1-3 pages:111-114
Abstract: The interaction of Na+ and K+ with proteins is at the basis of numerous processes of biological importance. However, measurement of the kinetic components of the interaction has eluded experimentalists for decades because the rate constants are too fast to resolve with conventional stopped-flow methods. Using a continuous-flow apparatus with a dead time of 50 mu s we have been able to resolve the kinetic rate constants and entire mechanism of Na+ binding to thrombin, an interaction that is at the basis of the procoagulant and prothrombotic roles of the enzyme in the blood. (c) 2007 Elsevier B.V All rights reserved.
ISSN: 0301-4622
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Genetics Section (-)
× corresponding author
# (joint) last author

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