Title: Depletion of Intracellular Ca2+ Stores Stimulates the Translocation of TRPV4-C1 Heteromeric Channels to the Plasma Membrane
Authors: Cao, Jingyuan ×
Luo, Jianhong
Nilius, Bernd
Huang, Yu
Ambudkar, Indu S
Yao, Xiaoqiang #
Issue Date: Nov-2010
Publisher: Lippincott, Williams & Wilkins
Series Title: Arteriosclerosis, Thrombosis and Vascular Biology vol:30 issue:11 pages:2249-2255
Abstract: OBJECTIVE: To examine the effect of Ca(2+) store depletion on the translocation of TRPV4-C1 heteromeric channels to the plasma membrane. METHODS AND RESULTS: Vesicular trafficking is a key mechanism for controlling the surface expression of TRP channels in the plasma membrane, where they perform their function. TRP channels in vivo are often composed of heteromeric subunits. Experiments using total internal fluorescence reflection microscopy and biotin surface labeling show that Ca(2+) store depletion enhanced TRPV4-C1 translocation into the plasma membrane in human embryonic kidney 293 cells that were coexpressed with TRPV4 and TRPC1. Fluorescent Ca(2+) measurement and patch clamp studies demonstrated that Ca(2+) store depletion enhanced 4alpha-PDD-stimulated Ca(2+) influx and cation current. The translocation required STIM1. TRPV4-C1 heteromeric channels were more favorably translocated to the plasma membrane than TRPC1 or TRPV4 homomeric channels. Similar results were obtained in native vascular endothelial cells. CONCLUSIONS: Ca(2+) store depletion stimulates the insertion of TRPV4-C1 heteromeric channels into the plasma membrane, resulting in an augmented Ca(2+) influx in response to flow in the human embryonic kidney cell overexpression system and native endothelial cells.
ISSN: 1079-5642
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Ion Channel Research
Department of Cellular and Molecular Medicine - miscellaneous
× corresponding author
# (joint) last author

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