Title: Growth and mitogenic effects of arylphorin in vivo and in vitro
Authors: Hakim, R S
Blackburn, M B
Corti, P
Gelman, D B
Goodman, C
Elsen, K
Loeb, M J
Lynn, D
Soin, T
Smagghe, G # ×
Issue Date: Feb-2007
Series Title: Archives of insect biochemistry and physiology vol:64 issue:2 pages:63-73
Abstract: In insects, developmental responses are organ- and tissue-specific. In previous studies of insect midgut cells in primary tissue cultures, growth-promoting and differentiation factors were identified from the growth media, hemolymph, and fat body. Recently, it was determined that the mitogenic effect of a Manduca sexta fat body extract on midgut stem cells of Heliothis virescens was due to the presence of monomeric alpha-arylphorin. Here we report that in primary midgut cell cultures, this same arylphorin stimulates stem cell proliferation in the lepidopterans M. sexta and Spodoptera littoralis, and in the beetle Leptinotarsa decemlineata. Studies using S. littoralis cells confirm that the mitogenic effect is due to free alpha-arylphorin subunits. In addition, feeding artificial diets containing arylphorin increased the growth rates of several insect species. When tested against continuous cell lines, including some with midgut and fat body origins, arylphorin had no effect; however, a cell line derived from Lymantria dispar fat body grew more rapidly in medium containing a chymotryptic digest of arylphorin.
ISSN: 0739-4462
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory for Mechanisms of Cell Transformation (VIB-KU Leuven Centre for Cancer Biology)
× corresponding author
# (joint) last author

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