Title: PEGylated lipids impede the lateral diffusion of adsorbed proteins at the surface of (magneto)liposomes
Authors: Nuytten, Nele
Hakimhashemi, M
Ysenbaert, Tine
Defour, L
Trekker, Jesse
Soenen, Stefaan
Van Der Meeren, Paul
De Cuyper, Marcel # ×
Issue Date: Oct-2010
Publisher: Elsevier
Series Title: Colloids and Surfaces B, Biointerfaces vol:80 issue:2 pages:227-231
Abstract: Protein binding to nanoparticles is a crucial issue in biomedicine, as it triggers their clearance from the bloodstream after intravenous injection. Many techniques are available for measuring strong protein binding interactions, but weak dynamic interactions are more difficult to assess. To tackle the latter problem, in the present work, cytochrome c was chosen as a representative model of a water-soluble protein and the adsorbing particulates were either small unilamellar phospholipid vesicles or 14 nm diameter solid superparamagnetic iron oxide cores onto which a phospholipid bilayer was strongly chemisorbed (so-called magnetoliposomes). Incorporation of cytochrome c oxidase into the phospholipid bilayer allowed the association of cytochrome c with the surface of the particles to be measured with high sensitivity by VIS-spectrophotometry. The impact of enzyme density as well as some of the physical features of the PEG corona (degree of PEGylation and PEG chain length) adjacent to the surface of the lipid structures on the overall kinetics was also investigated.
ISSN: 0927-7765
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Faculty of Medicine, Campus Kulak Kortrijk
Interdepartmental Platform Hospital Care
Biomedical MRI
Interdisciplinary Research Facility Life Sciences, Campus Kulak Kortrijk
× corresponding author
# (joint) last author

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