Title: Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters
Authors: Antoku, Yasuko ×
Hotta, Jun-ichi
Mizuno, Hideaki
Dickson, Robert M
Hofkens, Johan
Vosch, Tom #
Issue Date: 2010
Publisher: Royal soc chemistry
Series Title: Photochemical & photobiological sciences vol:9 issue:5 pages:716-721
Abstract: The fluorescence of silver clusters encapsulated by single stranded oligo-DNA (24 cytosine base pairs, C-24:Ag-n) was used to monitor the transfection of this new silver/DNA fluorophore inside living HeLa cells. For this, the C-24:Ag-n molecules were complexed with a commercially available transfection reagent Lipofectamine and the internalization of C-24:Ag-n was followed with confocal fluorescence microscopy. Bright near-infrared fluorescence was observed from inside the transfected HeLa cells, when exciting with 633 nm excitation, opening up the possibility for the use of these C-24:Ag-n clusters for biological labelling and imaging of living cells and for monitoring the transfection process with limited harm to the living cells.
ISSN: 1474-905X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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