Title: New PI(4,5)P-2- and membrane proximal integrin-binding motifs in the talin head control beta 3-integrin clustering
Authors: Saltel, Frederic
Mortier, Eva
Hytoenen, Vesa P
Jacquier, Marie-Claude
Zimmermann, Pascale
Vogel, Viola
Liu, Wei
Wehrle-Haller, Bernhard # ×
Issue Date: Nov-2009
Publisher: Rockefeller univ press
Series Title: Journal of cell biology vol:187 issue:5 pages:715-731
Abstract: Integrin-dependent adhesion sites consist of clustered integrins that transmit mechanical forces and provide signaling required for cell survival and morphogenesis. Despite their importance, the regulation of integrin clustering by the cytoplasmic adapter protein talin (Tal) and phosphatidylinositol (PI)-4,5-biphosphate (PI(4,5)P-2) lipids nor their dynamic coupling to the actin cytoskeleton is fully understood. By using a Tal-dependent integrin clustering assay in intact cells, we identified a PI(4,5)P-2-binding basic ridge spanning across the F2 and F3 domains of the Tal head that regulates integrin clustering. Clustering requires a new PI(4,5)P-2-binding site in F2 and is negatively regulated by autoinhibitory interactions between F3 and the Tal rod (Tal-R). The release of the Tal-R exposes a new beta 3-integrin-binding site in F3, enabling interaction with a membrane proximal acidic motif, which involves the formation of salt bridges between K-316 and K-324 with E-726 and D-723, respectively. This interaction shields the beta-integrin tail from reassociation with its beta subunit, thereby maintaining the integrin in a substrate-binding and clustering-competent form.
ISSN: 0021-9525
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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