Title: 2D-DIGE reveals changes in wheat xylanase inhibitor protein families due to grain development and Fusarium graminearum ∇Tri5 infection
Authors: Dornez, Emmie ×
Croes, Evi
Gebruers, Kurt
Carpentier, Sebastien
Swennen, Rony
Laukens, Kris
Witters, Erwin
Urban, Martin
Delcour, Jan
Courtin, Christophe #
Issue Date: Jun-2010
Publisher: WILEY-VCH Verlag
Series Title: Proteomics vol:10 issue:12 pages:2303-2319
Abstract: Wheat contains three different classes of proteinaceous xylanase inhibitors (XIs), i.e. Triticum aestivum xylanase inhibitors (TAXIs) xylanase-inhibiting proteins (XIPs), and thaumatin-like xylanase inhibitors (TLXIs) which are believed to act as a defensive barrier against phytopathogenic attack. In the absence of relevant data in wheat kernels, we here examined the response of the different members of the XI protein population to infection with a Delta Tri5 mutant of Fusarium graminearum, the wild type of which is one of the most important wheat ear pathogens, in early developing wheat grain. Wheat ears were inoculated at anthesis, analyzed using 2-D DICE and multivariate analysis at 5, 15, and 25 days post anthesis (DPA), and compared with control samples. Distinct abundance patterns could be distinguished for different XI forms in response to infection with F. graminearum Delta Tri5. Some (iso)forms were up-regulated, whereas others were down-regulated. This pathogen-specific regulation of proteins was mostly visible at five DPA and levelled off in the samples situated further from the inoculation point. Furthermore, it was shown that most identified TAXI- and XIP-type XI (iso)forms significantly increased in abundance from the milky (15 DPA) to the soft dough stages (25 DPA) on a per kernel basis, although the extent of increase differed greatly. Non-glycosylated XIP forms increased more strongly than their glycosylated counterparts.
ISSN: 1615-9853
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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