Title: Pituitary Progenitor Cells Tracked Down by Side Population Dissection
Authors: Chen, Jianghai
Gremeaux, Lies
Fu, Qiuli
Liekens, Daisy
Van Laere, Steven
Vankelecom, Hugo # ×
Issue Date: 2009
Publisher: Alphamed press
Series Title: Stem cells vol:27 issue:5 pages:1182-95
Abstract: Sca1(high) subset within the SP, stem cell growth factors that induce SP expansion, affect transcription of embryonic factors, suggesting impact on a developmental program that unfolds within this SP compartment. Non-Sca1(high) SP cells, revealed by Sox2 expression, are observed in the postulated periluminal stem/progenitor cell niche, but also in small groups scattered over the gland, thereby advocating the existence of multiple niches. In early postnatal mice undergoing a pituitary growth wave, Sox2 1 cells are more abundant than in adults, concordant with a larger SP and higher non-Sca1(high) proportion. Together, we tracked down pituitary progenitor cells by SP phenotype, and thus provide a straightforward method to isolate and scrutinize these cells from the plastic pituitary ex vivo, as well as a culture system for in-depth exploration of their regulatory network. STEM CELLS 2009;27:1182-1195The pituitary gland represents the endocrine core, governing the body's hormonal landscape by adapting its cellular composition to changing demands. It is assumed that stem/progenitor cells are involved in this remodeling. Recently, we uncovered a candidate stem/progenitor cell population in the anterior pituitary. Here, we scrutinized this "side population'' ( SP) and show that, unexpectedly, not the subset expressing high levels of "stem cell antigen-1'' ( Sca1(high)) but the remainder non-Sca1(high) fraction clusters the pituitary progenitor cells. Transcriptomal interrogation revealed in the non-Sca1(high) SP upregulated expression of the pituitary stem/progenitor cell markers Sox2 and Sox9, and of multiple factors critically involved in pituitary embryogenesis. The non-Sca1(high) SP encloses the cells that generate spheres and display multipotent hormone differentiation capacity. In culture conditions selecting for the non-
ISSN: 1066-5099
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Tissue Plasticity (-)
Pharmacology Section (-)
Section Woman - Miscellaneous (-)
Gynaecological Oncology
× corresponding author
# (joint) last author

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