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Title: The Dog Liver Contains a "Side Population" of Cells with Hepatic Progenitor-Like Characteristics
Authors: Arends, Brigitte ×
Vankelecom, Hugo
Borght, Sara Vander
Roskams, Tania
Penning, Louis C
Rothuizen, Jan
Spee, Bart #
Issue Date: Mar-2009
Publisher: Mary ann liebert inc
Series Title: Stem cells and development vol:18 issue:2 pages:343-350
Abstract: The aim of this study was to isolate and characterize potential progenitor cells from healthy dog livers. Stem/progenitor cells can be prospectively isolated from a diversity of tissues using their ability to efficiently pump out the dye Hoechst33342, thereby portraying a side population (SP) in dual-wavelength flow cytometry. We here describe the detection of a SP in dog liver, constituting similar to 3 % of the nonparenchymal-enriched cell fractions. A subpopulation of the SP (similar to 30 %) was immunonegative for the panhematopoietic marker CD45, and consisted predominantly of small, mononuclear, keratin 7-immunoreactive cells; characteristics suggestive of a liver progenitor cell phenotype. Both the CD45- and CD45+ SP showed upregulated expression of progenitor/cholangiocyte marker genes, but also low-level expression of hepatocyte markers, suggesting the presence of progenitor cells committed to the hepatic lineage in both SP fractions. Our findings demonstrate that healthy canine liver contains a small population of cells with progenitor-like characteristics that can be isolated on the basis of efficient Hoechst33342 expulsion.
ISSN: 1547-3287
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Tissue Plasticity (-)
Translational Cell & Tissue Research
× corresponding author
# (joint) last author

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