Title: Selective Nasal Allergen Provocation Induces Substance P-mediated Bronchial Hyperresponsiveness
Authors: Hens, Greet ×
Raap, Ulrike
Vanoirbeek, Jeroen
Meyts, Isabelle
Callebaut, Ina
Verbinnen, Bert
Vanaudenaerde, Bart
Cadot, Pascal
Nemery, Benoit
Bullens, Dominique
Ceuppens, Jan
Hellings, Peter #
Issue Date: Apr-2011
Publisher: American Lung Association
Series Title: American Journal of Respiratory Cell and Molecular Biology vol:44 issue:4 pages:517-523
Abstract: Rationale: Although the concept of âglobal airway allergyâ has become widely accepted during recent years, nasobronchial interaction and its mechanisms remain incompletely understood. The experimental study of the effect of nasal allergen deposition on lower airway pathology is hampered by the difficulty to avoid lower airway penetration of the allergens. Methods: In ovalbumin-sensitized mice with experimental airway allergy, nasal allergen provocations were performed after complete anatomical separation of upper and lower airways by means of a tracheotomy. A canula was inserted in the trachea and the trachea was ligated, thus inhibiting any passage of allergens from upper to lower airways. Results: Mice showed bronchial hyperresponsiveness to methacholine as early as 4 h after nasal allergen provocation in the absence of recruitment of inflammatory cells. An increased substance P concentration in the bronchial lumen was found, as well as an increased number of substance P-positive pulmonary nerves. Treatment with a neurokinin 1 receptor antagonist abolished the allergen-induced bronchial hyperresponsiveness. Moreover, endobronchial administration of substance P caused neurokinin 1 receptor-dependent bronchial hyperresponsiveness in mice with airway allergy. Conclusions: Nasal allergen provocation rapidly induces bronchial hyperresponsiveness via pulmonary upregulation of substance P and activation of NK 1 receptors.
ISSN: 1044-1549
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical Immunology
Research Group Experimental Oto-rhino-laryngology
Laboratory of Pediatric Immunology
Laboratory of Clinical Bacteriology and Mycology
Environment and Health - miscellaneous
Occupational, Environmental and Insurance Medicine (-)
Experimental Laboratory Immunology
Department of Neurosciences - miscellaneous
× corresponding author
# (joint) last author

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