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Title: Determination of 9-nitrocamptothecin and its metabolite 9-aminocamptothecin in human plasma using high-performance liquid chromatography with ultraviolet and fluorescence detection
Authors: Shoemaker, NE ×
Rosing, H
Jansen, S
Schöffski, Patrick
Rizzo, J
Schellens, JHM
Beijnen, JH #
Issue Date: Aug-2002
Publisher: Elsevier
Series Title: Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences vol:775 issue:2 pages:231-237
Abstract: A high-performance liquid chromatography assay is described for the determination of the investigational anti-cancer drug 9-nitrocamptothecin (9-NC) and its metabolite 9-aminocamptothecin (9-AC) as the total of their lactone and carboxylate forms. The sample pre-treatment consisted of a deproteinisation step and a quantitative acid-catalyzed conversion of all 9-NC and 9-AC into their lactone forms and a subsequent solid-phase extraction. Redissolved extracts were analyzed on a Prodigy analytical column, using a mixture of methanol-phosphate buffer (pH 2.5). Detection was concomitantly performed with UV for 9-NC and fluorimetrically for 9-AC. The lower limit of quantifications were 10 ng/ml and 2.5 ng/ml for the determination of 9-NC and 9-AC, respectively, using 500 mul of plasma. The presented method was successfully applied to a clinical pharmacokinetic study. (C) 2002 Elsevier Science B.V. All rights reserved.
ISSN: 1570-0232
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Experimental Oncology
Plant Systematics and Ecology Section - miscellaneous
× corresponding author
# (joint) last author

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