Title: Nonlinear optical properties of mStrawberry and mCherry for second harmonic imaging
Authors: De Meulenaere, Evelien
De Wergifosse, M.
Botek, E.
Spaepen, Stijn
Champagne, B.
Vanderleyden, Jozef
Clays, Koen # ×
Issue Date: 2010
Publisher: World Scientific
Series Title: Journal of Nonlinear Optical Physics & Materials vol:19 issue:1 pages:1-13
Abstract: The second-order nonlinear optical properties of two monomeric red fluorescent proteins, mStrawberry and mCherry, have been experimentally determined by frequency-resolved femtosecond hyper-Rayleigh scattering. These proteins were found to exhibit a stronger nonlinear response than the previously described eGFP, eYFP and DsRed,(1) confirming the trend that fluorophores with a more extended conjugated system, or a lower-energy band gap between ground and excited state, exhibit a larger static hyperpolarizability (beta(0)). Furthermore, these experimental data were complemented with quantum chemical calculations. A discrepancy was observed between experimental and theoretical results, but this could be explained by the chromophore model extracted from the available X-ray diffraction data. While eGFP showed a larger dynamic experimental response (beta(HRS)) due to the highest resonance enhancement, we measured an even higher signal for mCherry. Furthermore, mCherry also shows a better separation of the second harmonic signal and two-photon excited fluorescent signal, making this the preferred fluorescent protein for second harmonic imaging at 800nm so far.
ISSN: 0218-8635
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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