Title: Cardioselective nitric oxide synthase 3 gene transfer protects against myocardial reperfusion injury
Authors: Szelid, Zsolt ×
Pokreisz, Peter
Liu, Xiaoshun
Vermeersch, Pieter
Marsboom, Glenn
Gillijns, Hilde
Pellens, Marijke
Verbeken, Erik
Van de Werf, Frans
Collen, Desire
Janssens, Stefan P #
Issue Date: Mar-2010
Publisher: Dr dietrich steinkopff verlag
Series Title: Basic research in cardiology vol:105 issue:2 pages:169-179
Abstract: Nitric oxide modulates the severity of myocardial ischemia-reperfusion (I/R) injury. We investigated whether cardioselective nitric oxide synthase 3 (NOS3) gene transfer could confer myocardial protection against I/R injury in pigs and examined potential molecular mechanisms. I/R injury was induced by balloon occlusion of the left anterior descending artery for 45 min followed by 4 or 72 h reperfusion. Hemodynamic and pathological changes were measured in pigs in the absence (n = 11) or presence of prior intracoronary retroinfusion of human NOS3 (AdNOS3, 5 x 10(10) PFU, n = 13) or control vector (AdRR5, 5 x 10(10) PFU, n = 11). Retrograde NOS3 gene transfer selectively increased NOS3 expression and NO bioavailability in the area at risk (AAR) without changing endogenous NOS isoform expression. At 4 h R, LV systolic (dP/dt (max)) and diastolic (dP/dt (min)) function was better preserved in AdNOS3- than in AdRR5-injected pigs (2,539 +/- A 165 vs. 1,829 +/- A 156 mmHg/s, and -2,781 +/- A 340 vs. -2,062 +/- A 292 mmHg/s, respectively, P < 0.05 for both). Myocardial infarct size (% AAR) was significantly smaller in AdNOS3 than in control and AdRR5 and associated with a significantly greater reduction in cardiac myeloperoxidase activity, a marker of neutrophil infiltration. The latter effects were sustained at 72 h R in a subset of pigs (n = 7). In the AAR, intercellular endothelial adhesion molecule-1 expression and cardiomyocyte apoptosis were significantly lower in AdNOS3. In conclusion, single myocardial NOS3 retroinfusion attenuates I/R injury, and causes a sustained reduction in myocardial infarct size and inflammatory cell infiltration. Gene-based strategies to increase NO bioavailability may have therapeutic potential in myocardial I/R.
ISSN: 0300-8428
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Cardiology
Molecular and Vascular Biology
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science