Title: Spectroscopic Rationale for Efficient Stimulated-Emission Depletion Microscopy Fluorophores
Authors: Hotta, Jun-ichi ×
Fron, Eduard
Dedecker, Peter
Janssen, Kris Pieter Frans
Muellen, Klaus
Harke, Benjamin
Bueckers, Johanna
Hell, Stefan W
Hofkens, Johan #
Issue Date: Apr-2010
Publisher: Amer chemical soc
Series Title: Journal of the american chemical society vol:132 issue:14 pages:5021-+
Abstract: We report a rationale for identifying superior dyes for stimulated-emission depletion (STED) microscopy. We compared the dyes pPDI and pTDI, which displayed excellent photostability in single-molecule spectroscopy. Surprisingly, their photostability and performance in STED microscopy differed significantly. While single pTDI molecules could be visualized with excellent resolution (35 nm), pPDI molecules bleached rapidly under similar conditions. Femtosecond transient absorption measurements proved that the overlap between the stimulated-emission band and the excited-state absorption band is the main reason for the observed difference. Thus, assessment of the excited-state absorption band provides a rational means of dye selection and determination of the optimal wavelength for STED.
ISSN: 0002-7863
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Mechatronics, Biostatistics and Sensors (MeBioS)
Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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