Title: Loss or Silencing of the PHD1 Prolyl Hydroxylase Protects Livers of Mice Against Ischemia/Reperfusion Injury
Authors: Schneider, Martin ×
Van Geyte, Katie
Fraisl, Peter
Kiss, Judit
Aragones, Julian
Mazzone, Massimiliano
Mairbaeurl, Heimo
De Bock, Katrien
Jeoung, Nam Ho
Mollenhauer, Martin
Georgiadou, Maria
Bishop, Tammie
Roncal, Carmen
Sutherland, Andrew
Jordan, Benedicte
Gallez, Bernard
Weitz, Juergen
Harris, Robert A
Maxwell, Patrick
Baes, Myriam
Ratcliffe, Peter
Carmeliet, Peter #
Issue Date: Mar-2010
Publisher: W b saunders co-elsevier inc
Series Title: Gastroenterology vol:138 issue:3 pages:1143-U438
Abstract: BACKGROUND & AIMS: Liver ischemia/reperfusion (I/R) injury is a frequent cause of organ dysfunction. Loss of the oxygen sensor prolyl hydroxylase domain enzyme 1 (PHD1) causes tolerance of skeletal muscle to hypoxia. We assessed whether loss or short-term silencing of PHD1 could likewise induce hypoxia tolerance in hepatocytes and protect them against hepatic I/R damage. METHODS: Hepatic ischemia was induced in mice by clamping of the portal vessels of the left lateral liver lobe; 90 minutes later livers were reperfused for 8 hours for I/R experiments. Hepatocyte damage following ischemia or I/R was investigated in PHD1-deficient (PHD1(-/-)) and wild-type mice or following short hairpin RNA-mediated short-term inhibition of PHD1 in vivo. RESULTS: PHD1(-/-) livers were largely protected against acute ischemia or I/R injury. Among mice subjected to hepatic I/R followed by surgical resection of all nonischemic liver lobes, more than half of wild-type mice succumbed, whereas all PHD1(-/-) mice survived. Also, short-term inhibition of PHD1 through RNA interference-mediated silencing provided protection against I/R. Knockdown of PHD1 also induced hypoxia tolerance of hepatocytes in vitro. Mechanistically, loss of PHD1 decreased production of oxidative stress, which likely relates to a decrease in oxygen consumption as a result of a reprogramming of hepatocellular metabolism. CONCLUSIONS: Loss of PHD1 provided tolerance of hepatocytes to acute hypoxia and protected them against I/R-damage. Short-term inhibition of PHD1 is a novel therapeutic approach to reducing or preventing I/R-induced liver injury.
ISSN: 0016-5085
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Cell Metabolism
Vesalius Research Centre (-)
Laboratory of Tumor Inflammation and Angiogenesis (Vesalius Research Center) (+)
Laboratory of Angiogenesis and Vascular Metabolism (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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