Title: Calcium phosphate functionalisation of Ti6Al4V scaffolds by perfusion electrodeposition
Authors: Chai, Yoke Chin
Vleugels, Jef
Luyten, Frank
Schrooten, Jan #
Issue Date: 19-Apr-2010
Host Document: Materials for Sustainable Production
Conference: Innovation for Sustainable Production 2010 location:Brugge, Belgium date:19 - 22 April 2010
Article number: page 34
Abstract: Introduction: Functionalisation of titanium (Ti) implants with calcium phosphate (CaP) is a widely used approach in
orthopaedic applications to obtain a mechanically strong system that is
Osteoconductive . In order to deposit CaP in a controlled way on complex 3D Ti surfaces, like scaffolds, this study applied
a perfusion electrodeposition (P-ELD) system.
Materials and Methods: 3D Ti-scaffolds were fabricated by selective laser melting. A P-ELD system was developed
to deposit CaP onto Ti-scaffolds (cathode) using a platinum ring as anode. A supersaturated calcium phosphate (SCP)
solution was used as electrolyte. A full factorial (24) design was performed to analyse the effect of current density (I),
temperature (T), deposition time (t) and flow rate (f) on the characteristics of the deposited CaP. The parameter I and t
were optimised by performing P-ELD at: (a) varying I at constant t (12 hr) and (ii) varying t at constant I (3 mA/cm2). The
coating morphology, distribution, thickness, crystalinity and Ca/P ratio were characterised by scanning electron microscopy
(SEM), X-ray diffraction (XRD) and electron probe micro- analysis (EPMA). The biocompatibility of CaP-coated
Ti-scaffolds was tested by live-dead staining and SEM-analysis of human periosteum derived cells (HPDCs) seeded
scaffolds after 7 days of culture.
Results and Discussion: CaP deposition increased with increasing t, T and f, whereas 20 & 40 mA/cm2 of I were too
high and disrupted CaP deposition. In fact, the effect of t and the t-f interaction on the CaP deposition were statistically
significant (p=0.001 & p=0.019). 50 oC and 10 ml/min were selected for subsequent experiments. SEM analysis showed
that P-ELD for t > 6 hr with 2 – 10 mA/cm2 resulted in a full coating of the scaffolds, up to a thickness of 40μm and with
a Ca/P ratio of 1.41. Interestingly, P-ELD at 5 mA/cm2 for 6 hr produced a cauli flower-like crystal structure of 28 μm
thick with a Ca/P ratio of 1.45. XRD analysis indicated that the CaP coatings were carbonated synthetic hydroxyapatite.
Live-dead staining of HPDCs cultured on coated Ti-scaffolds for 7 days showed high cell viability and biocompatibility.
SEM imaging showed that the HPDCs had a fibroblastic phenotype and interacted with the CaP coating.
Conclusion: Perfusion electrodeposition (P-ELD) can become a useful tool to functionalise complex Ti structures (e.g.
scaffolds) with CaP, in which the physiochemical properties of the CaP coating could be controlled and optimised for
effective bone formation.
Publication status: published
KU Leuven publication type: AMa
Appears in Collections:Rheumatology Section (-)
Physical Metallurgy and Materials Engineering Section (-)
Department of Materials Engineering - miscellaneous
# (joint) last author

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