Title: Loss of high-frequency glucose-induced Ca2+ oscillations in pancreatic islets correlates with impaired glucose tolerance in Trpm5(-/-) mice
Authors: Colsoul, Barbara *
Schraenen, Anica *
Lemaire, Katleen
Quintens, Roel
Van Lommel, Leentje
Segal, Andrei
Owsianik, Grzegorz
Talavera PĂ©rez, Karel
Voets, Thomas
Margolskee, Robert F
Kokrashvili, Zaza
Gilon, Patrick
Nilius, Bernd
Schuit, Frans
Vennekens, Rudi # ×
Issue Date: Mar-2010
Publisher: Natl acad sciences
Series Title: Proceedings of the national academy of sciences of the united states of america vol:107 issue:11 pages:5208-5213
Abstract: Glucose homeostasis is critically dependent on insulin release from pancreatic beta-cells, which is strictly regulated by glucose-induced oscillations in membrane potential (Vm) and the cytosolic calcium level ([Ca2+](cyt)). We propose that TRPM5, a Ca2+-activated monovalent cation channel, is a positive regulator of glucose-induced insulin release. Immunofluorescence revealed expression of TRPM5 in pancreatic islets. A Ca2+-activated nonselective cation current with TRPM5-like properties is significantly reduced in Trpm(5-/-) cells. Ca2+-imaging and electrophysiological analysis show that glucose- induced oscillations of V-m and [Ca2+](cyt) have on average a reduced frequency in Trpm5(-/-)islets, specifically due to a lack of fast oscillations. As a consequence, glucose-induced insulin release from Trpm5(-/-) pancreatic islets is significantly reduced, resulting in an impaired glucose tolerance in Trpm5(-/-) mice.
ISSN: 0027-8424
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Ion Channel Research
Gene Expression Unit
Department of Cellular and Molecular Medicine - miscellaneous
* (joint) first author
× corresponding author
# (joint) last author

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