Title: Plant pectin methylesterase and its inhibitor from kiwi fruit: Interaction analysis by surface plasmon resonance
Authors: Jolie, Ruben
Duvetter, Thomas
Houben, Ken
Vandevenne, Evelien
Van Loey, Ann
Declerck, Paul
Hendrickx, Marc
Gils, Ann # ×
Issue Date: Jul-2010
Publisher: Elsevier sci ltd
Series Title: Food chemistry vol:121 issue:1 pages:207-214
Abstract: Two surface plasmon resonance (SPR)-based interaction analysis methods were Successfully implemented to explore the binding between plant PME and kiwi PMEI. In a first method, plant PMEs were immobilised on a chip surface via amine Coupling. This experimental setup allowed studying the effect of pH and ionic strength on the PME-PMEI interaction kinetics. Strong binding was obtained at pH < 7 and at low salt concentrations, whereas both pH >= 8 and [NaCl] of ca. 1.0 M effectively caused dissociation. In a second method, kiwi PMEI was immobilised on a chip surface to which streptavidin had been covalently attached. Hereto, PMEI was biotinylated by means of a NHS-biotin reagent. With this immobilisation strategy, the effect of (partial) thermal or high pressure-induced denaturation of PME on its affinity towards PMEI was investigated. A notable degree of enzyme inactivation was required before interaction characteristics were significantly altered. Any incomplete inactivation of PME resulted in binding to the PMEI Surface. (C) 2009 Elsevier Ltd. All rights reserved,
ISSN: 0308-8146
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory for Pharmaceutical Biology (-)
Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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