Title: Isolation procedure and characterization of multipotent adult progenitor cells from rat bone marrow
Authors: Subramanian, Kartik ×
Geraerts, Martine
Pauwelyn, Karen
Park, Yonsil
Owens, D Jason
Muijtjens, Manja
Ulloa-Montoya, Fernando
Jiang, Yeuhua
Verfaillie, Catherine
Hu, Wei-Shou #
Issue Date: 2010
Series Title: Methods in Molecular Biology vol:636 pages:55-78
Abstract: Multipotent adult progenitor cells (MAPCs) are adult stem cells derived from the bone marrow of mouse and rat and were described for the first time in 2002 (Jiang et al., Nature 418:41-49, 2002), and subsequently (Breyer et al., Exp Hematol 34:1596-1601, 2006; Jiang et al., Exp Hematol 30:896-904, 2002; Ulloa-Montoya et al., Genome Biol 8:R163, 2007). The capacity of rodent MAPC to differentiate at the single-cell level into some of the cell types of endoderm, mesoderm, and neuroectoderm germ layer lineages makes them promising candidates for the study of developmental processes. MAPC are isolated using adherent cell cultures and are selected based on morphology after a period of about 8-18 weeks. Here, we describe a step-by-step reproducible method to isolate rat MAPC from fetal and adult bone marrow. We elaborate on several aspects of the isolation protocol including, cell density and medium components, and methods for selecting and obtaining potential MAPC clones and their characterization.
ISSN: 1064-3745
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Hepatology
Interdepartemental Stem Cell Institute (-)
× corresponding author
# (joint) last author

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