Title: Identification of Small Molecule Inhibitors of Salmonella Biofilm Formation and Investigation of Their Mode of Action
Authors: Steenackers, Hans
Janssens, J.
Levin, Jeremy
De Vos, Dirk
Vanderleyden, Jozef
De Keersmaecker, Sigrid #
Issue Date: 21-Apr-2009
Host Document: Young Microbiologists Mini-Symposium on 'Microbe signalling, organization and pathogenesis', UCC, Cork, 2009
Conference: Young Microbiologists Mini-Symposium on 'Microbe signalling, organization and pathogenesis' edition:1 location:Cork, Ireland date:21-22 April 2009
Abstract: Salmonella is worldwide one of the most important foodborne pathogens. A major difficulty in the control of Salmonella infections is the fact that Salmonella can form multicellular structures, commonly called biofilms, on various biotic and abiotic surfaces. Within these biofilms, Salmonella is protected against a wide range of environmental influences such as antibiotics, disinfectants and the immune system. Therefore, the prevention or eradication of these biofilms could be an effective way to restrict the spread of Salmonella. Importantly, molecules that inhibit the biofilm formation do not necessarily kill the bacteria. In this way, the development of resistance, which is a major drawback of classical antibiotics, is less likely. As such, biofilm inhibitors are a sustainable alternative in the production of safe and healthy food.
Our quest for powerful biofilm inhibitors is based on two strategies. In the first strategy, we study the influence of several synthetic analogues of the halogenated furanones on Salmonella biofilms. The halogenated furanones, a class of molecules which were originally derived from the red alga Delisea pulchra, were previously shown to interfere with the quorum sensing systems and the biofilm formation of several bacterial pathogens. We demonstrated the strong inhibitory effect of the halogenated furanones on Salmonella biofilm formation and delineated a broad structure-activity-relationship. Currently, we are investigating the exact mode of action of the halogenated furanones inhibiting Salmonella biofilm formation by using a broad scope of techniques such as gene reporter fusions, transcriptome analysis and screening of a mutant library. In the second strategy, we use a number of ligand-based and receptor-based computational techniques to identify new biofilm inhibitors. By this approach, we have discovered a powerful class of inhibitors, of which we currently are investigating the molecular mode of action.
Publication status: published
KU Leuven publication type: IMa
Appears in Collections:Centre of Microbial and Plant Genetics
Centre for Surface Chemistry and Catalysis
# (joint) last author

Files in This Item:

There are no files associated with this item.


All items in Lirias are protected by copyright, with all rights reserved.