Title: Self-complementary sequence context in mature miRNAs
Authors: Maiti, Mohitosh
Nauwelaerts, Koen
Lescrinier, Eveline
Schuit, Frans
Herdewijn, Piet # ×
Issue Date: Feb-2010
Series Title: Biochemical and biophysical research communications vol:392 issue:4 pages:572-576
Abstract: MicroRNAs (miRNAs) are a class of 19-25 nt long non-coding RNAs that regulate gene expression post-transcriptionally by binding with partially complementary sequences in the 3'-untranslated region (3'-UTR) and inhibiting mRNA translation or by affecting mRNA stability. We have characterized the structures and the equilibrium between hairpin and homo-duplex form of the mature strand of hsa-mir-520h by various concentration and temperature dependent 1D, 2D NMR experiments and those structures correspond well with Mfold-folded and UNAFold-aligned secondary structures. A detailed folding and alignment analysis in physiological conditions of all mature miRNA strands from the complete database of known miRNAs (miRBase) was performed. The statistical analysis of the resulting folding and alignment data showed for the first time the potential of a large number of mature miRNAs to form significant hairpin and/or homo-duplex structures in solution. The self-complementarity of mature miRNAs can provide a mechanistic tuning and a regulatory sophistication to the process of miRNA mediated gene regulation.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
Gene Expression Unit
× corresponding author
# (joint) last author

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