KVCV - VJC10 location:Blankenberge, Belgium date:1-2 March 2010
Bone Tissue Engineering (TE) is an emerging interdisciplinary field applying the principles of biology and engineering, by combining bone forming cells together with a biocompatible carrier structure, to the development of viable bone substitutes that restore and maintain the function of human bone tissues.
Recent research shows that cell source, density and distribution appeared to be essential in optimization of the first step of TE in which cells are involved, namely the seeding of the cell into the carrier structure.
In the presented research, cells are applied and incubated on an inert titanium scaffold. Hereafter cell seeding efficiency is determined by DNA-content measurements. Different cell sources are tested in an optimized seeding procedure: an osteosarcoma cell line is used for proof of principle as opposed to human periosteum derived cells which are clinically relevant.
Optimized cell seeding aims to relate cell seeding efficiency to objective parameters, such as cell source, scaffold morphology, cell concentration or available free volume. This ultimately being predictive for the functionality of an in vivo bone tissue engineered construct.