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Title: Biodistribution and Pharmacokinetics of PEG-10kDa-Cholecystokinin-10 in Rats after Different Routes of Administration
Authors: León-Tamariz, Fabián
Verbaeys, Isabelle
Van Boven, Maurits ×
De Cuyper, Marcel
Buyse, Johan
de Witte, Peter
Verbruggen, Alfons
Cokelaere, Marnix #
Issue Date: Apr-2010
Publisher: Bentham Science Publishers
Series Title: Current Drug Delivery vol:7 issue:2 pages:137-143
Abstract: Cholecystokinin, produced in the proximal small intestine, is a short acting satiating peptide hormone. CCK-10, before and after mono-iodination, was previously coupled to 10kDa polyethylene glycol (PEG). The formed conjugates PEG10kDa-CCK-10 and PEG10kDa-[(127)I]-CCK-10 show after i.p. administration to rats a sustained food intake reduction during 8h in comparison to 2h for free CCK-10. The present study examined the blood pharmacokinetics of this pharmacological interesting molecule by means of PEG10kDa-[(123)I]-CCK-10 following intravenous, intraperitoneal, intramuscular and nasal administration and the biodistribution after i.p. administration. HPLC analysis with radiometric detection allowed the differentiation between inorganic iodide and the intact tracer in blood. Blood kinetics after i.v. injection was fitted to a bi-exponential with a distribution half-life of 15 min and with an elimination half-life of 8 hours for intact PEG10kDa-[(123)I]-CCK-10. The biodistribution studies showed a higher accumulation of the tracer for all administration routes in organs expressing CCK receptors localized in the gastrointestinal tract such as pancreas, duodenum and small intestine. No indication of blood brain barrier crossing for the conjugate could be observed independently of the administration route. Main clearance was via the urinary pathway.
ISSN: 1567-2018
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Interdisciplinary Research Facility Life Sciences @ Kulak
Anatomy, Kulak (-)
Toxicology and Pharmacology
Division of Livestock-Nutrition-Quality
Laboratory for Pharmaceutical Biology (-)
Radiopharmacy
× corresponding author
# (joint) last author

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