Title: Synthesis and Characterization of Stable Monodisperse Silica Nanoparticle Sols for in Vitro Cytotoxicity Testing
Authors: Thomassen, Leen ×
Aerts, Alexander
Rabolli, Virginie
Lison, Dominique
Gonzalez, Laetitia
Kirsch-Volders, Micheline
Napierska, Dorota
Kirschhock, Christine
Martens, Johan #
Issue Date: Jan-2010
Publisher: American Chemical Society
Series Title: Langmuir vol:26 issue:1 pages:328-335
Abstract: For the investigation of the interaction of nanoparticles with biomolecules, cells, organs, and animal models there is a need for well-characterized nanoparticle suspensions. In this paper we report the preparation of monodisperse dense amorphous silica nanoparticles (SNP) suspended in physiological media that are sterile and sufficiently stable against aggregation. SNP sols with various particle sizes (2-335 nm) were prepared via base-catalyzed hydrolysis and polymerization of tetraethyl orthosilicate under sterile conditions using either ammonia (Stober process (1) ) or lysine catalyst (Lys-Sil process (2) ). The series was complemented with commercial silica sols (Ludox). Silica nanoparticle suspensions were purified by dialysis and dispersed without using any dispersing agent into cell culture media (Dulbecco's Modified Eagle's medium) containing antibiotics. Particle sizes were determined by dynamic light scattering. SNP morphology, surface area, and porosity were characterized using electron microscopy and nitrogen adsorption. The SNP sols in cell culture medium were stable for several days. The catalytic activity of the SNP in the conversion of hydrogen peroxide into hydroxyl radicals was investigated using electron paramagnetic resonance. The catalytic activity per square meter of exposed silica surface area was found to be independent of particle size and preparation method. Using this unique series of nanoparticle suspensions, the relationship between cytotoxicity and particle size was investigated using human endothelial and mouse monocyte-macrophage cells. The cytotoxicity of the SNP was strongly dependent on particle size and cell type. This unique methodology and the collection of well-characterized SNP will be useful for further in vitro studies exploring the physicochemical determinants of nanoparticle toxicity.
ISSN: 0743-7463
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Environment and Health - miscellaneous
Centre for Surface Chemistry and Catalysis
Occupational, Environmental and Insurance Medicine (-)
Formerly "Departement Industriƫle Wetenschappen - TM M"
Microbial and Molecular Systems - miscellaneous
× corresponding author
# (joint) last author

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