Title: Facile preparation of Langmuir-Blodgett films of water-soluble proteins and hybrid protein-clay films
Authors: Miao, Shiding ×
Leeman, Hugo
De Feyter, Steven
Schoonheydt, Robert #
Issue Date: 2010
Publisher: Royal soc chemistry
Series Title: Journal of materials chemistry vol:20 issue:4 pages:698-705
Abstract: Water-soluble protein monolayers have been prepared by spreading protein (lysozyme (Lys) and bovine serum albumin (BSA)) aqueous solutions over water and diluted clay (saponite) dispersions in a Langmuir-Blodgett (LB) trough. LB films of protein and hybrid protein-clay were prepared by vertical upstroke deposition at a desired surface pressure. Surface pressure-time (pi-t) curves and surface pressure-area isotherms (pi-A) indicate that the equilibrium time between the injection and compression plays an important role in forming a protein monolayer. Atomic force microscopy (AFM) suggests that heterogeneous films, consisting of regions of protein clusters and regions of saponite layers covered with protein clusters, are obtained. Both lysozyme and BSA accumulate particularly well at the edges of the saponite layers. The main difference is that the positively charged lysozyme is much more efficient in attracting negatively charged saponite layers at the air-water interface. The amount of lysozyme immobilized (n(S)) is 0.2-0.4 ng mm(-2) for the water-lysozyme film and 0.5-0.6 ng mm(-2) for the saponite-lysozyme film, as determined using UV-Vis spectroscopy. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) confirmed the presence of clay and proteins in the hybrid LB films. No significant change in the position of amide I or II bands was observed, suggesting little or no conformational changes upon immobilization of the proteins.
ISSN: 0959-9428
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Surface Chemistry and Catalysis
Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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