Title: Protein-based indicator system for detection of temperature differences in high pressure high temperature processing
Authors: Grauwet, Tara ×
Van der Plancken, Iesel
Vervoort, Liesbeth
Hendrickx, Marc
Van Loey, Ann #
Issue Date: Apr-2010
Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science
Series Title: Food Research International vol:43 pages:862-871
Abstract: Since the kinetics of change of target attributes in high pressure high temperature (HPHT) processing are
not only pressure but also clearly temperature dependent, a control of temperature in space and time is
indispensible. A tool that can easily detect temperature differences in prototype HPHT vessels during a
HPHT process would aid the design of industrial HPHT equipments. In this work, the potential of ovomucoid
as extrinsic, isolated, protein-based indicator to detect temperature differences inside a HPHT vessel
was assessed. Ovomucoid fulfilled the selection criteria of a candidate indicator for HPHT. Solvent engineering
was successfully used to shift the inactivation window of the candidate indicator into the HPHT
processing window. Since, the dissociation equilibrium of buffer systems shifts under high pressure and/
or under high temperature, a critical evaluation of the effect of a pH-shift of the selected phosphate buffer
system in the context of protein-based indicator development was performed. The ovomucoid system
inactivation kinetics were characterized under isobaric–isothermal conditions and modeled best by a first
order inactivation model. A temperature dependent inactivation at constant pressure could be observed,
which creates potential for the detection of temperature differences under HPHT conditions. These integrating
properties were unaffected under dynamic, industrially relevant HPHT conditions.
ISSN: 0963-9969
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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